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Characterization of GABA receptors.


ABSTRACT: Described in this unit are ligand-binding assays for GABAA , GABAB , and the homomeric ? GABAA (formerly GABAC ) receptor recognition sites in brain tissue. Although GABA binding sites are present in peripheral organs, most research is directed toward examining these receptors in the CNS. These assays may also be used to determine the affinity of an unlabeled compound for the GABA binding sites. Excluded from the unit are ligand-binding assays for other components of the GABAA receptor complex, such as the benzodiazepine or ion-channel binding sites.

SUBMITTER: Enna SJ 

PROVIDER: S-EPMC3927317 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Characterization of GABA receptors.

Enna S J SJ   McCarson Kenneth E KE  

Current protocols in pharmacology 20131202


Described in this unit are ligand-binding assays for GABAA , GABAB , and the homomeric ρ GABAA (formerly GABAC ) receptor recognition sites in brain tissue. Although GABA binding sites are present in peripheral organs, most research is directed toward examining these receptors in the CNS. These assays may also be used to determine the affinity of an unlabeled compound for the GABA binding sites. Excluded from the unit are ligand-binding assays for other components of the GABAA receptor complex,  ...[more]

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