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MiR-146a promotes the initiation and progression of melanoma by activating Notch signaling.


ABSTRACT: Oncogenic mutations in BRAF and NRAS occur in 70% of melanomas. In this study, we identify a microRNA, miR-146a, that is highly upregulated by oncogenic BRAF and NRAS. Expression of miR-146a increases the ability of human melanoma cells to proliferate in culture and form tumors in mice, whereas knockdown of miR-146a has the opposite effects. We show these oncogenic activities are due to miR-146a targeting the NUMB mRNA, a repressor of Notch signaling. Previous studies have shown that pre-miR-146a contains a single nucleotide polymorphism (C>G rs2910164). We find that the ability of pre-miR-146a/G to activate Notch signaling and promote oncogenesis is substantially higher than that of pre-miR-146a/C. Analysis of melanoma cell lines and matched patient samples indicates that during melanoma progression pre-miR-146a/G is enriched relative to pre-miR-146a/C, resulting from a C-to-G somatic mutation in pre-miR-146a/C. Collectively, our results reveal a central role for miR-146a in the initiation and progression of melanoma. DOI: http://dx.doi.org/10.7554/eLife.01460.001.

SUBMITTER: Forloni M 

PROVIDER: S-EPMC3927633 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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miR-146a promotes the initiation and progression of melanoma by activating Notch signaling.

Forloni Matteo M   Dogra Shaillay Kumar SK   Dong Yuying Y   Conte Darryl D   Ou Jianhong J   Zhu Lihua Julie LJ   Deng April A   Mahalingam Meera M   Green Michael R MR   Wajapeyee Narendra N  

eLife 20140218


Oncogenic mutations in BRAF and NRAS occur in 70% of melanomas. In this study, we identify a microRNA, miR-146a, that is highly upregulated by oncogenic BRAF and NRAS. Expression of miR-146a increases the ability of human melanoma cells to proliferate in culture and form tumors in mice, whereas knockdown of miR-146a has the opposite effects. We show these oncogenic activities are due to miR-146a targeting the NUMB mRNA, a repressor of Notch signaling. Previous studies have shown that pre-miR-146  ...[more]

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