Proofreading of noncognate acyl adenylates by an acyl-coenzyme a ligase.
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ABSTRACT:
SUBMITTER: Manandhar M
PROVIDER: S-EPMC3927786 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Publications
Proofreading of noncognate acyl adenylates by an acyl-coenzyme a ligase.
Manandhar Miglena M Cronan John E JE
Chemistry & biology 20131121 12
Aminoacyl-tRNA synthetases remove (proofread) incorrect substrates and thereby prevent errors in protein synthesis. We report enzyme-catalyzed pretransfer editing by pimeloyl-coenzyme A (CoA) ligase (BioW), a biotin synthetic enzyme that converts pimelate, a seven-carbon dicarboxylic acid, to its CoA ester. The noncognate BioW substrate glutaric acid results in hydrolysis of ATP to AMP with formation of only trace amounts of glutaryl-CoA, thereby mimicking pretransfer editing of incorrect aminoa ...[more]
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