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Genome characterization of Long Island tick rhabdovirus, a new virus identified in Amblyomma americanum ticks.

ABSTRACT: Ticks are implicated as hosts to a wide range of animal and human pathogens. The full range of microbes harbored by ticks has not yet been fully explored.As part of a viral surveillance and discovery project in arthropods, we used unbiased high-throughput sequencing to examine viromes of ticks collected on Long Island, New York in 2013.We detected and sequenced the complete genome of a novel rhabdovirus originating from a pool of Amblyomma americanum ticks. This virus, which we provisionally name Long Island tick rhabdovirus, is distantly related to Moussa virus from Africa.The Long Island tick rhabdovirus may represent a novel species within family Rhabdoviridae.

SUBMITTER: Tokarz R

PROVIDER: S-EPMC3928085 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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Genome characterization of Long Island tick rhabdovirus, a new virus identified in Amblyomma americanum ticks.

Tokarz Rafal R Sameroff Stephen S Leon Maria Sanchez MS Jain Komal K Lipkin W Ian WI

Virology journal 20140211

<h4>Background</h4>Ticks are implicated as hosts to a wide range of animal and human pathogens. The full range of microbes harbored by ticks has not yet been fully explored.<h4>Methods</h4>As part of a viral surveillance and discovery project in arthropods, we used unbiased high-throughput sequencing to examine viromes of ticks collected on Long Island, New York in 2013.<h4>Results</h4>We detected and sequenced the complete genome of a novel rhabdovirus originating from a pool of Amblyomma ameri ...[more]

PMID: 24517260

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Project description:The lone star tick (Amblyomma americanum) is an abundant and aggressive biter of humans, domestic animals, and wildlife in the southeastern-central USA and an important vector of several known and suspected zoonotic bacterial pathogens. However, the biological drivers of bacterial community variation in this tick are still poorly defined. Knowing the community context in which tick-borne bacterial pathogens exist and evolve is required to fully understand the ecology and immunobiology of the ticks and to design effective public health and veterinary interventions. We performed a metagenomic survey of the bacterial communities of questing A. americanum and tested 131 individuals (66 nymphs, 24 males, and 41 females) from five sites in three states. Pyrosequencing was performed with barcoded eubacterial primers targeting variable 16S rRNA gene regions 5-3. The bacterial communities were dominated by Rickettsia (likely R. amblyommii) and an obligate Coxiella symbiont, together accounting for 6.7-100% of sequences per tick. DNAs from Midichloria, Borrelia, Wolbachia, Ehrlichia, Pseudomonas, or unidentified Bacillales, Enterobacteriaceae, or Rhizobiales groups were also detected frequently. Wolbachia and Midichloria significantly co-occurred in Georgia (p<0.00001), but not in other states. The significance of the Midichloria-Wolbachia co-occurrence is unknown. Among ticks collected in Georgia, nymphs differed from adults in both the composition (p?=?0.002) and structure (p?=?0.002) of their bacterial communities. Adults differed only in their community structure (p?=?0.002) with males containing more Rickettsia and females containing more Coxiella. Comparisons among adult ticks collected in New York and North Carolina supported the findings from the Georgia collection despite differences in geography, collection date, and sample handling, implying that the differences detected are consistent attributes. The data also suggest that some members of the bacterial community change during the tick life cycle and that some sex-specific attributes may be detectable in nymphs.

Dataset Information

Genome characterization of Long Island tick rhabdovirus, a new virus identified in Amblyomma americanum ticks.

Publications

Genome characterization of Long Island tick rhabdovirus, a new virus identified in Amblyomma americanum ticks.

Similar Datasets

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