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In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes.

ABSTRACT: Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchoring in the liposomal membrane. Here, we show that the addition of TATp to DNA-loaded liposomes increased the uptake of DNA in DC. DNA-loaded TATp-L increased the in vitro transfection efficiency in DC cultures as evidenced by a higher expression of the enhanced green fluorescent protein and bovine herpes virus type 1 glycoprotein D (gD). The de novo synthesized gD protein was immunologically stimulating when transfections were performed with TATp-L, as indicated by the secretion of interleukin 6.

SUBMITTER: Pappalardo JS

PROVIDER: S-EPMC3928453 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes.

Pappalardo Juan Sebastián JS Langellotti Cecilia A CA Di Giacomo Sebastián S Olivera Valeria V Quattrocchi Valeria V Zamorano Patricia I PI Hartner William C WC Levchenko Tatyana S TS Torchilin Vladimir P VP

International journal of nanomedicine 20140213

Dendritic cells (DC) are antigen-presenting cells uniquely capable of priming naïve T cells and cross-presenting antigens, and they determine the type of immune response elicited against an antigen. TAT peptide (TATp), is an amphipathic, arginine-rich, cationic peptide that promotes penetration and translocation of various molecules and nanoparticles into cells. TATp-liposomes (TATp-L) used for DC transfection were prepared using TATp derivatized with a lipid-terminated polymer capable of anchor ...[more]

PMID: 24611012

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In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes.

Publications

In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes.

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