Ontology highlight
ABSTRACT:
SUBMITTER: Song W
PROVIDER: S-EPMC3929357 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Song Wenjiao W Strack Rita L RL Svensen Nina N Jaffrey Samie R SR
Journal of the American Chemical Society 20140118 4
Spinach and Spinach2 are RNA aptamers that can be used for the genetic encoding of fluorescent RNA. Spinach2 binds and activates the fluorescence of (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one (DFHBI), allowing the dynamic localizations of Spinach2-tagged RNAs to be imaged in live cells. The spectral properties of Spinach2 are limited by DFHBI, which produces fluorescence that is bluish-green and is not optimized for filters commonly used in fluorescence microsco ...[more]