Ontology highlight
ABSTRACT:
SUBMITTER: Kiffer-Moreira T
PROVIDER: S-EPMC3933536 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Kiffer-Moreira Tina T Sheen Campbell R CR Gasque Kellen Cristina da Silva KC Bolean Mayte M Ciancaglini Pietro P van Elsas Andrea A Hoylaerts Marc F MF Millán José Luis JL
PloS one 20140224 2
Recombinant alkaline phosphatases are becoming promising protein therapeutics to prevent skeletal mineralization defects, inflammatory bowel diseases, and treat acute kidney injury. By substituting the flexible crown domain of human intestinal alkaline phosphatase (IAP) with that of the human placental isozyme (PLAP) we generated a chimeric enzyme (ChimAP) that retains the structural folding of IAP, but displays greatly increased stability, active site Zn²⁺ binding, increased transphosphorylatio ...[more]