Unknown

Dataset Information

0

Polymeric micelles for apoptosis-targeted optical imaging of cancer and intraoperative surgical guidance.


ABSTRACT: In a two-step strategy, an intraperitoneal (IP) injection of poly(ethylene glycol)-block-poly(ε-caprolactone) (PEG-b-PCL) micelles containing paclitaxel (PTX), cyclopamine (CYP), and gossypol (GSP) at 30, 30, and 30 mg/kg, respectively, debulked tumor tissues by 1.3-fold, based on loss of bioluminescence with <10% body weight change, and induced apoptosis in peritoneal tumors when used as neoadjuvant chemotherapy (NACT) in an ES-2-luc-bearing xenograft model for ovarian cancer. In a second step, a single intravenous (i.v.) injection of apoptosis-targeting GFNFRLKAGAKIRFGS-PEG-b-PCL micelles containing a near-infrared (NIR) fluorescence probe, DiR (1,1'-dioctadecyltetramethyl indotricarbocyanine iodide), resulted in increased peritoneal DiR accumulation in apoptosis-induced ES-2-luc tumor tissues (ex vivo) by 1.5-fold compared with DiR molecules delivered by methoxy PEG-b-PCL micelles (non-targeted) at 48 h after i.v. injection in a second step. As a result, a tandem of PEG-b-PCL micelles enabled high-resolution detection of ca. 1 mm diameter tumors, resulting in resection of approximately 90% of tumors, and a low peritoneal cancer index (PCI) of ca. 7. Thus, a tandem of PEG-b-PCL micelles used for NCAT and NIR fluorescence imaging of therapy-induced apoptosis for intraoperative surgical guidance may be a promising treatment strategy for metastatic ovarian cancer.

SUBMITTER: Cho H 

PROVIDER: S-EPMC3935963 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3463236 | biostudies-literature
| S-EPMC4829353 | biostudies-literature
| S-EPMC7261260 | biostudies-literature
| S-EPMC3844036 | biostudies-literature
| S-EPMC4643322 | biostudies-literature
| S-EPMC4938571 | biostudies-literature
| S-EPMC6831285 | biostudies-literature
| S-EPMC2542943 | biostudies-literature
| S-EPMC6266309 | biostudies-other
| S-EPMC4330038 | biostudies-literature