Ontology highlight
ABSTRACT:
SUBMITTER: Thyme SB
PROVIDER: S-EPMC3936771 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Thyme Summer B SB Boissel Sandrine J S SJ Arshiya Quadri S S Nolan Tony T Baker Dean A DA Park Rachel U RU Kusak Lara L Ashworth Justin J Baker David D
Nucleic acids research 20131121 4
Homing endonucleases (HEs) can be used to induce targeted genome modification to reduce the fitness of pathogen vectors such as the malaria-transmitting Anopheles gambiae and to correct deleterious mutations in genetic diseases. We describe the creation of an extensive set of HE variants with novel DNA cleavage specificities using an integrated experimental and computational approach. Using computational modeling and an improved selection strategy, which optimizes specificity in addition to acti ...[more]