Dataset Information

GPR30 activation decreases anxiety in the open field test but not in the elevated plus maze test in female mice.

ABSTRACT: The GPR30 is a novel estrogen receptor (ER) that is a candidate membrane ER based on its binding to 17? estradiol and its rapid signaling properties such as activation of the extracellular-regulated kinase (ERK) pathway. Its distribution in the mouse limbic system predicts a role for this receptor in the estrogenic modulation of anxiety behaviors in the mouse. A previous study showed that chronic administration of a selective agonist to the GPR30 receptor, G-1, in the female rat can improve spatial memory, suggesting that GPR30 plays a role in hippocampal-dependent cognition. In this study, we investigated the effect of a similar chronic administration of G-1 on behaviors that denote anxiety in adult ovariectomized female mice, using the elevated plus maze (EPM) and the open field test as well as the activation of the ERK pathway in the hippocampus. Although estradiol benzoate had no effect on behaviors in the EPM or the open field, G-1 had an anxiolytic effect solely in the open field that was independent of ERK signaling in either the ventral or dorsal hippocampus. Such an anxiolytic effect may underlie the ability of G-1 to increase spatial memory, by acting on the hippocampus.

SUBMITTER: Anchan D

PROVIDER: S-EPMC3937706 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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GPR30 activation decreases anxiety in the open field test but not in the elevated plus maze test in female mice.

Anchan Divya D Clark Sara S Pollard Kevin K Vasudevan Nandini N

Brain and behavior 20131127 1

The GPR30 is a novel estrogen receptor (ER) that is a candidate membrane ER based on its binding to 17β estradiol and its rapid signaling properties such as activation of the extracellular-regulated kinase (ERK) pathway. Its distribution in the mouse limbic system predicts a role for this receptor in the estrogenic modulation of anxiety behaviors in the mouse. A previous study showed that chronic administration of a selective agonist to the GPR30 receptor, G-1, in the female rat can improve spat ...[more]

PMID: 24653954

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Project description:The elevated plus maze test is a widely used test for assessing anxiety-like behavior and screening novel therapeutic agents in rodents. Previous studies have shown that a variety of internal factors and procedural variables can influence elevated plus maze behavior. Although some studies have suggested a link between behavior and plasma corticosterone levels, the relationships between them remain unclear. In this study, we investigated the effects of experience with a battery of behavioral tests, the wall color of the closed arms, and illumination level on the behavior and plasma corticosterone responses in the elevated plus maze in male C57BL/6J mice. Mice were either subjected to a series of behavioral tests, including assessments of general health and neurological function, a light/dark transition test, and an open field test, or left undisturbed until the start of the elevated plus maze test. The mice with and without test battery experience were allowed to freely explore the elevated plus maze. The other two independent groups of naïve mice were tested in mazes with closed arms with different wall colors (clear, transparent blue, white, and black) or different illumination levels (5, 100, and 800 lx). Immediately after the test, blood was collected to measure plasma corticosterone concentrations. Mice with test battery experience showed a lower percentage of open arm time and entries and, somewhat paradoxically, had lower plasma corticosterone levels than the mice with no test battery experience. Mice tested in the maze with closed arms with clear walls exhibited higher open arm exploration than mice tested in the maze with closed arms with black walls, while there were no significant differences in plasma corticosterone levels between the different wall color conditions. Illumination levels had no significant effects on any measure. Our results indicate that experience with other behavioral tests and different physical features of the maze affect elevated plus maze behaviors. Increased open arm time and entries are conventionally interpreted as decreased anxiety-like behavior, while other possible interpretations are considered: open arm exploration may reflect heightened anxiety and panic-like reaction to a novel situation under certain conditions. With the possibility of different interpretations, the present findings highlight the need to carefully consider the test conditions in designing experiments and drawing conclusions from the behavioral outcomes in the elevated plus maze test in C57BL/6J mice.

Project description:Tail biting is a serious welfare concern in pig production. It not only causes distress for victims, but may occur where pigs are unable to cope, and become biters. An animal's ability to cope with stressful situations may vary between individuals, but the behavioral response could be consistent across different fear eliciting situations. We exposed 75 pigs to open field (OF) and novel object (NO) tests at 14 weeks of age. Within each pen of pigs (n = 16 pens, 55 pigs/pen), 6 pigs were selected for testing using the following criteria: 3 pigs that had severe bite wounds (BITTEN), 1 confirmed biter (BITER), 1 pig which could be easily approached and trained to provide a saliva sample (BOLD) and 1 pig which was extremely evasive, and was unable to be trained to willingly provide a saliva sample (SHY). Given that responses may be consistent in different scenarios, we hypothesized that SHY pigs would display more characteristics of a fear response (i.e., less movement in the open field, more time spent by the door, and longer latency to approach the novel object) than human BOLD pigs. We also hypothesized that BITTEN pigs would behave similarly to SHY and BITERS similarly to BOLD. The BOLD and BITER pigs spent more time exploring (P < 0.05) and less time by the door (P < 0.01) than the BITTEN and SHY pigs. Although there was an overall increase in cortisol level from before to after the tests (P < 0.001), this was only significant for BITTEN (P < 0.001) and SHY (P < 0.05) pigs. Therefore, as hypothesized, for several measures, BOLD, and BITER pigs behaved similarly, and differently to SHY and BITTEN. However, the low sample size potentially meant that for several measures, although numeric differences were in the direction hypothesized, there were no statistical differences. Further work in which a greater number of BITER pigs were included in the sample, may elucidate our hypotheses more clearly, as to whether responses to fear tests in pigs could be associated with the likelihood of being a tail biter, or victim.

Dataset Information

GPR30 activation decreases anxiety in the open field test but not in the elevated plus maze test in female mice.

Publications

GPR30 activation decreases anxiety in the open field test but not in the elevated plus maze test in female mice.

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