Immobilization of homogeneous monomeric, oligomeric and fibrillar A? species for reliable SPR measurements.
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ABSTRACT:
SUBMITTER: Frenzel D
PROVIDER: S-EPMC3940443 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
Immobilization of homogeneous monomeric, oligomeric and fibrillar Aβ species for reliable SPR measurements.
Frenzel Daniel D Glück Julian M JM Brener Oleksandr O Oesterhelt Filipp F Nagel-Steger Luitgard L Willbold Dieter D
PloS one 20140303 3
There is strong evidence that the amyloid-beta peptide (Aβ) plays a central role in the pathogenesis of Alzheimer's disease (AD). In this context, a detailed quantitative description of the interactions with different Aβ species is essential for characterization of physiological and artificial ligands. However, the high aggregation propensity of Aβ in concert with its susceptibility to structural changes due to even slight changes in solution conditions has impeded surface plasmon resonance (SPR ...[more]
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