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Mononuclear cells from a rare blood donor, after freezing under good manufacturing practice conditions, generate red blood cells that recapitulate the rare blood phenotype.

ABSTRACT: Cultured red blood cells (cRBCs) from cord blood (CB) have been proposed as transfusion products. Whether buffy coats discarded from blood donations (adult blood [AB]) may be used to generate cRBCs for transfusion has not been investigated.Erythroid progenitor cell content and numbers and blood group antigen profiles of erythroblasts (ERYs) and cRBCs generated in human erythroid massive amplification (HEMA) culture by CB (n?=?7) and AB (n?=?33, three females, three males, one AB with rare blood antigens cryopreserved using CB protocols) were compared.Variability was observed both in progenitor cell content (twofold) and number of ERYs generated (1?log) by CB and AB in HEMA. The average progenitor cell contents of the subset of AB and CB analyzed were similar. AB generated numbers of ERYs three times lower (p?

SUBMITTER: Masiello F 

PROVIDER: S-EPMC3942379 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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Mononuclear cells from a rare blood donor, after freezing under good manufacturing practice conditions, generate red blood cells that recapitulate the rare blood phenotype.

Masiello Francesca F   Tirelli Valentina V   Sanchez Massimo M   van den Akker Emile E   Gabriella Girelli G   Marconi Maurizio M   Villa Maria Antonietta MA   Rebulla Paolo P   Hashmi Ghazala G   Whitsett Carolyn C   Migliaccio Anna Rita AR  

Transfusion 20130904 4

<h4>Background</h4>Cultured red blood cells (cRBCs) from cord blood (CB) have been proposed as transfusion products. Whether buffy coats discarded from blood donations (adult blood [AB]) may be used to generate cRBCs for transfusion has not been investigated.<h4>Study design and methods</h4>Erythroid progenitor cell content and numbers and blood group antigen profiles of erythroblasts (ERYs) and cRBCs generated in human erythroid massive amplification (HEMA) culture by CB (n = 7) and AB (n = 33,  ...[more]

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