Exploiting cell-to-cell variability to detect cellular perturbations.
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ABSTRACT:
SUBMITTER: Dey G
PROVIDER: S-EPMC3942435 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
Exploiting cell-to-cell variability to detect cellular perturbations.
Dey Gautam G Gupta Gagan D GD Ramalingam Balaji B Sathe Mugdha M Mayor Satyajit S Thattai Mukund M
PloS one 20140304 3
Any single-cell-resolved measurement generates a population distribution of phenotypes, characterized by a mean, a variance, and a shape. Here we show that changes in the shape of a phenotypic distribution can signal perturbations to cellular processes, providing a way to screen for underlying molecular machinery. We analyzed images of a Drosophila S2R+ cell line perturbed by RNA interference, and tracked 27 single-cell features which report on endocytic activity, and cell and nuclear morphology ...[more]
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