Unknown

Dataset Information

0

Integrated proteomic analysis of post-translational modifications by serial enrichment.

ABSTRACT: We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.

SUBMITTER: Mertins P 

PROVIDER: S-EPMC3943163 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Integrated proteomic analysis of post-translational modifications by serial enrichment.

Mertins Philipp P   Qiao Jana W JW   Patel Jinal J   Udeshi Namrata D ND   Clauser Karl R KR   Mani D R DR   Burgess Michael W MW   Gillette Michael A MA   Jaffe Jacob D JD   Carr Steven A SA  

Nature methods 20130609 7

We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exe  ...[more]