Unknown

Dataset Information

0

Hexane Fractions of Bupleurum falcatum L. Stimulates Glucagon-Like Peptide-1 Secretion through G ? ? -Mediated Pathway.

ABSTRACT: Bupleurum falcatum L. has been used traditionally as a medicinal herb in Korean medicine. The hexane fraction of BF (HFBF), which was profiled with Direct Analysis in Real Time-Mass Spectrometry (DART-MS), activates the secretion of glucagon-like peptide-1 (GLP-1) in NCI-H716 cells significantly. We performed a microarray analysis and GLP-1 ELISA assay, as well as calcium imaging experiments with inhibitors, to investigate the mechanism of action of the HFBF. Through the microarray analysis, it was found that the ITPR2 gene that encodes the inositol 1,4,5-trisphosphate (IP3) receptor is up-regulated and the HFBF induces cell depolarization by inhibiting the voltage-gated channel expression in NCI-H716 cells. In addition, we found that the intracellular calcium in NCI-H716 cells, with Gallein, U73122, and 2APB as inhibitors, was decreased. These results suggest that the HFBF activates the GLP-1 secretion through the G ?? pathways in the enteroendocrine L cells after treatment with the HFBF.

SUBMITTER: Shin MH 

PROVIDER: S-EPMC3943199 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Hexane Fractions of Bupleurum falcatum L. Stimulates Glucagon-Like Peptide-1 Secretion through G β γ -Mediated Pathway.

Shin Min-Hee MH   Choi Eun-Kyeong EK   Kim Ki-Suk KS   Kim Kang-Hoon KH   Jang Young Pyo YP   Ahn Kwang Seok KS   Chung Won-Seok WS   Cha Nam Hyun NH   Jang Hyeung-Jin HJ  

Evidence-based complementary and alternative medicine : eCAM 20140213

Bupleurum falcatum L. has been used traditionally as a medicinal herb in Korean medicine. The hexane fraction of BF (HFBF), which was profiled with Direct Analysis in Real Time-Mass Spectrometry (DART-MS), activates the secretion of glucagon-like peptide-1 (GLP-1) in NCI-H716 cells significantly. We performed a microarray analysis and GLP-1 ELISA assay, as well as calcium imaging experiments with inhibitors, to investigate the mechanism of action of the HFBF. Through the microarray analysis, it  ...[more]

Similar Datasets

Genomics Bupleurum falcatum
| PRJNA413106 | ENA
Unknown Glucagon-like peptide 1 stimulates post-translational activation of glucokinase in pancreatic beta cells.
| S-EPMC3089519 | biostudies-literature
Unknown Novel Small Molecule Glucagon-Like Peptide-1 Receptor Agonist S6 Stimulates Insulin Secretion From Rat Islets.
| S-EPMC8116734 | biostudies-literature
Unknown Ghrelin directly stimulates glucagon secretion from pancreatic alpha-cells.
| S-EPMC3165914 | biostudies-literature
Unknown The complete mitochondrial genome sequences of <i>Bupleurum falcatum</i> (Apiales: Apiaceae).
| S-EPMC7782287 | biostudies-literature
Unknown Beta-Arrestin-1 mediates glucagon-like peptide-1 signaling to insulin secretion in cultured pancreatic beta cells.
| S-EPMC2373360 | biostudies-literature
Unknown The Complete Chloroplast Genome of the Hare's Ear Root, Bupleurum falcatum: Its Molecular Features.
| S-EPMC4880840 | biostudies-literature
Unknown Activation of intestinal olfactory receptor stimulates glucagon-like peptide-1 secretion in enteroendocrine cells and attenuates hyperglycemia in type 2 diabetic mice.
| S-EPMC5656655 | biostudies-literature
Genomics Nobiletin ameliorates high fat-induced disruptions in rhythmic glucagon-like peptide-1 secretion - 16S
2022-05-06 | E-MTAB-11551 | biostudies-arrayexpress
Unknown Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16? hydroxylase from Bupleurum falcatum.
| S-EPMC3910630 | biostudies-literature