Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega-3 fatty acids.

ABSTRACT: In contrast to well-characterized PUFA levels in serum, little is known regarding their downstream metabolic products. However, many of these compounds are lipid mediators with prominent roles during pro- and antiinflammatory processes.In this double blind crossover study on asthmatics, shifts in serum levels of ?-3 and ?-6 PUFA-derived oxidized fatty acids (e.g. eicosanoids, oxylipins) were quantified following dietary fish oil supplementation. Serum was obtained from subjects following fasting at three occasions; (i) prior to supplementation, (ii) following a 3-week supplement intake of either placebo or fish oil, and (iii) following a 3-week washout period with a subsequent 3-week period of either fish oil or placebo supplement. A total of 87 oxylipins representing cyclooxygenase (COX), lipoxygenase (LOX), and cytochrome P450 (CYP) metabolic pathways were screened via LC-MS/MS. The primary alterations observed were in CYP- and 15-LOX-derived EPA- and CYP-derived DHA oxylipins.The results indicate that intake of an ?-3 rich diet alters not only the PUFA ratio, but also the ratio of downstream oxylipins. These data further support that dietary manipulation with ?-3 PUFAs affects not only PUFA levels, but importantly also the downstream metabolic profile.

SUBMITTER: Lundstrom SL 

PROVIDER: S-EPMC3943463 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature