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Correlative super-resolution fluorescence and metal-replica transmission electron microscopy.

ABSTRACT: We combine super-resolution localization fluorescence microscopy with transmission electron microscopy of metal replicas to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. We validate robust correlation on the scale of 20 nm by imaging endogenous clathrin (in two and three dimensions) and apply the method to find the previously unknown three-dimensional position of the endocytic protein epsin on clathrin-coated structures at the plasma membrane.

SUBMITTER: Sochacki KA

PROVIDER: S-EPMC3943662 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Correlative super-resolution fluorescence and metal-replica transmission electron microscopy.

Sochacki Kem A KA Shtengel Gleb G van Engelenburg Schuyler B SB Hess Harald F HF Taraska Justin W JW

Nature methods 20140126 3

We combine super-resolution localization fluorescence microscopy with transmission electron microscopy of metal replicas to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. We validate robust correlation on the scale of 20 nm by imaging endogenous clathrin (in two and three dimensions) and apply the method to find the previously unknown three-dimensional position of the endocytic protein epsin on clathrin-coated structures at the plasma membrane. ...[more]

PMID: 24464288

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