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Crystallization and preliminary X-ray diffraction analysis of the interleukin-3 alpha receptor bound to the Fab fragment of antibody CSL362.

ABSTRACT: Interleukin-3 (IL-3) is a member of the beta common family of cytokines that regulate multiple functions of myeloid cells. The IL-3 receptor-specific alpha subunit (IL3R?) is overexpressed on stem cells/progenitor cells of patients with acute myeloid leukaemia, where elevated receptor expression correlates clinically with a reduced patient survival rate. The monoclonal antibody (MAb) CSL362 is a humanized MAb derived from the murine MAb 7G3, originally identified for its ability to specifically recognize the human IL-3 receptor and for blocking the signalling of IL-3 in myeloid and endothelial cells. In order to elucidate the molecular mechanism of CSL362 antagonism, a preliminary structure of human IL3R? in complex with the MAb CSL362 has been determined.

SUBMITTER: Broughton SE 

PROVIDER: S-EPMC3944702 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray diffraction analysis of the interleukin-3 alpha receptor bound to the Fab fragment of antibody CSL362.

Broughton Sophie E SE   Hercus Timothy R TR   Nero Tracy L TL   Dhagat Urmi U   Owczarek Catherine M CM   Hardy Matthew P MP   Fabri Louis J LJ   Scotney Pierre D PD   Nash Andrew D AD   Wilson Nicholas J NJ   Lopez Angel F AF   Parker Michael W MW  

Acta crystallographica. Section F, Structural biology communications 20140219 Pt 3

Interleukin-3 (IL-3) is a member of the beta common family of cytokines that regulate multiple functions of myeloid cells. The IL-3 receptor-specific alpha subunit (IL3Rα) is overexpressed on stem cells/progenitor cells of patients with acute myeloid leukaemia, where elevated receptor expression correlates clinically with a reduced patient survival rate. The monoclonal antibody (MAb) CSL362 is a humanized MAb derived from the murine MAb 7G3, originally identified for its ability to specifically  ...[more]

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