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Substrates with patterned extracellular matrix and subcellular stiffness gradients reveal local biomechanical responses.

ABSTRACT: A substrate fabrication process is developed to pattern both the extracellular matrix (ECM) and rigidity at sub-cellular spatial resolution. When growing cells on these substrates, it is found that cells respond locally in their cytoskeleton assembly. The presented method allows unique insight into the biological interpretation of mechanical signals, whereas photolithography-based fabrication is amenable to integration with complex microfabricated substructures.

SUBMITTER: Tseng P

PROVIDER: S-EPMC3947434 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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Substrates with patterned extracellular matrix and subcellular stiffness gradients reveal local biomechanical responses.

Tseng Peter P Di Carlo Dino D

Advanced materials (Deerfield Beach, Fla.) 20131209 8

A substrate fabrication process is developed to pattern both the extracellular matrix (ECM) and rigidity at sub-cellular spatial resolution. When growing cells on these substrates, it is found that cells respond locally in their cytoskeleton assembly. The presented method allows unique insight into the biological interpretation of mechanical signals, whereas photolithography-based fabrication is amenable to integration with complex microfabricated substructures. ...[more]

PMID: 24323894

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