Ontology highlight
ABSTRACT:
SUBMITTER: Nakayama T
PROVIDER: S-EPMC3947545 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Nakayama Takuya T Fish Margaret B MB Fisher Marilyn M Oomen-Hajagos Jamina J Thomsen Gerald H GH Grainger Robert M RM
Genesis (New York, N.Y. : 2000) 20131201 12
We have assessed the efficacy of the recently developed CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system for genome modification in the amphibian Xenopus tropicalis. As a model experiment, targeted mutations of the tyrosinase gene were verified, showing the expected albinism phenotype in injected embryos. We further tested this technology by interrupting the six3 gene, which is required for proper eye and brain formation. Expected eye and brain phen ...[more]