The human parvovirus B19 non-structural protein 1 N-terminal domain specifically binds to the origin of replication in the viral DNA.
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ABSTRACT:
SUBMITTER: Tewary SK
PROVIDER: S-EPMC3953040 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
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The human parvovirus B19 non-structural protein 1 N-terminal domain specifically binds to the origin of replication in the viral DNA.
Tewary Sunil Kumar SK Zhao Haiyan H Deng Xuefeng X Qiu Jianming J Tang Liang L
Virology 20131220
The non-structural protein 1 (NS1) of human parvovirus B19 plays a critical role in viral DNA replication. Previous studies identified the origin of replication in the viral DNA, which contains four DNA elements, namely NSBE1 to NSBE4, that are required for optimal viral replication (Guan et al., 2009). Here we have demonstrated in vitro that the NS1 N-terminal domain (NS1N) binds to the origin of replication in a sequence-specific, length-dependent manner that requires NSBE1 and NSBE2, while NS ...[more]
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