Dataset Information

Interactome analysis of AMP-activated protein kinase (AMPK)-?1 and -?1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.

ABSTRACT: The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in regulation and pathogenesis, there are limited data on the interaction of protein kinases. To identify proteins that interact with AMPK, we performed large-scale affinity purification (AP)-mass spectrometry (MS) of the AMPK-?1 and -?1 subunits. Through a comprehensive analysis, using a combination of immunoprecipitaion and ion trap mass spectrometry, we identified 381 unique proteins in the AMPK?/? interactomes: 325 partners of AMPK-?1 and 243 for AMPK-?1. Further, we identified 196 novel protein-protein interactions with AMPK-?1 and AMPK-?1. Notably, in our bioinformatics analysis, the novel interaction partners mediated functions that are related to the regulation of actin organization. Specifically, several such proteins were linked to pancreatic beta-cell functions, including glucose-stimulated insulin secretion, beta-cell development, beta-cell differentiation, and cell-cell communication.

SUBMITTER: Moon S

PROVIDER: S-EPMC3953747 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.

Moon Sungyoon S Han Dohyun D Kim Yikwon Y Jin Jonghwa J Ho Won-Kyung WK Kim Youngsoo Y

Scientific reports 20140314

The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in regulation and pathogenesis, there are limited data on the interact ...[more]

PMID: 24625528

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Dataset Information

Interactome analysis of AMP-activated protein kinase (AMPK)-?1 and -?1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.

Publications

Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.

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