Ontology highlight
ABSTRACT:
SUBMITTER: Zheng Q
PROVIDER: S-EPMC3959289 | biostudies-literature | 2014 Mar-Apr
REPOSITORIES: biostudies-literature
Photochemistry and photobiology 20131202 2
Photoexcitation of fluorophores commonly used for biological imaging applications generates reactive oxygen species (ROS) which can cause bleaching of the fluorophore and damage to the biological system under investigation. In this study, we show that singlet oxygen contributes relatively little to Cy5 and ATTO 647N photobleaching at low concentrations in aqueous solution. We also show that Cy5 generates significantly less ROS when covalently linked to the protective agents, cyclooctatetraene (C ...[more]