Ontology highlight
ABSTRACT:
SUBMITTER: Baker P
PROVIDER: S-EPMC3962088 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Baker Perrin P Seah Stephen Y K SY
Computational and structural biotechnology journal 20121002
Enzymes that catalyze carbon-carbon bond formation can be exploited as biocatalyst for synthetic organic chemistry. However, natural enzymes frequently do not possess the required properties or specificities to catalyze industrially useful transformations. This mini-review describes recent work using knowledge-guided site-specific mutagenesis of key active site residues to alter substrate specificity, stereospecificity and reaction specificity of these enzymes. In addition, examples of de novo d ...[more]