Ontology highlight
ABSTRACT:
SUBMITTER: Schmuck EG
PROVIDER: S-EPMC3964807 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Schmuck Eric G EG Mulligan Jacob D JD Ertel Rebecca L RL Kouris Nicholas A NA Ogle Brenda M BM Raval Amish N AN Saupe Kurt W KW
Cardiovascular engineering and technology 20140301 1
<h4>Purpose</h4>Demonstrate a novel manufacturing method to generate extracellular matrix scaffolds from cardiac fibroblasts (CF-ECM) as a therapeutic mesenchymal stem cell-transfer device.<h4>Materials and methods</h4>Rat CF were cultured at high-density (~1.6×10<sup>5</sup>/cm<sup>2</sup>) for 10-14 days. Cell sheets were removed from the culture dish by incubation with EDTA and decellularized with water and peracetic acid. CF-ECM was characterized by mass spectrometry, immunofluorescence and ...[more]