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Imported case of acute respiratory tract infection associated with a member of species nelson bay orthoreovirus.

ABSTRACT: A Japanese man suffered from acute respiratory tract infection after returning to Japan from Bali, Indonesia in 2007. Miyazaki-Bali/2007, a strain of the species of Nelson Bay orthoreovirus, was isolated from the patient's throat swab using Vero cells, in which syncytium formation was observed. This is the sixth report describing a patient with respiratory tract infection caused by an orthoreovirus classified to the species of Nelson Bay orthoreovirus. Given the possibility that all of the patients were infected in Malaysia and Indonesia, prospective surveillance on orthoreovirus infections should be carried out in Southeast Asia. Furthermore, contact surveillance study suggests that the risk of human-to-human infection of the species of Nelson Bay orthoreovirus would seem to be low.

SUBMITTER: Yamanaka A

PROVIDER: S-EPMC3965453 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Imported case of acute respiratory tract infection associated with a member of species nelson bay orthoreovirus.

Yamanaka Atsushi A Iwakiri Akira A Yoshikawa Tomoki T Sakai Kouji K Singh Harpal H Himeji Daisuke D Kikuchi Ikuo I Ueda Akira A Yamamoto Seigo S Miura Miho M Shioyama Yoko Y Kawano Kimiko K Nagaishi Tokiko T Saito Minako M Minomo Masumi M Iwamoto Naoyasu N Hidaka Yoshio Y Sohma Hirotoshi H Kobayashi Takeshi T Kanai Yuta Y Kawagishi Takehiro T Nagata Noriyo N Fukushi Shuetsu S Mizutani Tetsuya T Tani Hideki H Taniguchi Satoshi S Fukuma Aiko A Shimojima Masayuki M Kurane Ichiro I Kageyama Tsutomu T Odagiri Takato T Saijo Masayuki M Morikawa Shigeru S

PloS one 20140325 3

A Japanese man suffered from acute respiratory tract infection after returning to Japan from Bali, Indonesia in 2007. Miyazaki-Bali/2007, a strain of the species of Nelson Bay orthoreovirus, was isolated from the patient's throat swab using Vero cells, in which syncytium formation was observed. This is the sixth report describing a patient with respiratory tract infection caused by an orthoreovirus classified to the species of Nelson Bay orthoreovirus. Given the possibility that all of the patie ...[more]

PMID: 24667794

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Project description:BackgroundNelson Bay orthoreovirus (NBV) is a fusogenic bat borne virus with an unknown zoonotic potential. Previous studies have shown that NBV can infect and replicate in a wide variety of cell types derived from their natural host (bat), as well as from human, mouse and monkey. Within permissive cells, NBV induced significant cytopathic effects characterised by cell-cell fusion and syncytia formation. To understand the molecular events that underpin NBV infection we examined the host transcriptome and proteome response of two cell types, derived from bat (PaKiT03) and mouse (L929), to characterise differential cellular susceptibility to NBV.ResultsDespite significant differences in NBV replication and cytopathic effects in the L929 and PaKiT03 cells, the host response was remarkably similar in these cells. At both the transcriptome and proteome level, the host response was dominated by IFN production and signalling pathways. The majority of proteins up-regulated in L929 and PaKiT03 cells were also up-regulated at the mRNA (gene) level, and included many important IFN stimulated genes. Further functional experimentation demonstrated that stimulating IFN signalling prior to infection, significantly reduced NBV replication in PaKiT03 cells. Moreover, inhibiting IFN signalling (through specific siRNAs) increased NBV replication in L929 cells. In line with the significant cytopathic effects seen in PaKiT03 cells, we also observed a down-regulation of genes involved in cell-cell junctions, which may be related to the fusogenic effects of NBV.ConclusionsThis study provides new multi-dimensional insights into the host response of mammalian cells to NBV infection. We show that IFN activity is capable of reducing NBV replication, although it is unlikely that this is solely responsible for the reduced replication of NBV in L929 cells. The molecular events that underpin the fusogenic cytopathic effects described here will prove valuable for identifying potential therapeutic targets against fusogenic orthoreovirus.

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Imported case of acute respiratory tract infection associated with a member of species nelson bay orthoreovirus.

Publications

Imported case of acute respiratory tract infection associated with a member of species nelson bay orthoreovirus.

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