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Snail modulates the assembly of fibronectin via ?5 integrin for myocardial migration in zebrafish embryos.

ABSTRACT: The Snail family member snail encodes a zinc finger-containing transcriptional factor that is involved in heart formation. Yet, little is known about how Snail regulates heart development. Here, we identified that one of the duplicated snail genes, snai1b, was expressed in the heart region of zebrafish embryos. Depletion of Snai1b function dramatically reduced expression of ?5 integrin, disrupted Fibronectin layer in the heart region, especially at the midline, and prevented migration of cardiac precursors, resulting in defects in cardiac morphology and function in zebrafish embryos. Injection of ?5?1 protein rescued the Fibronectin layer and then the myocardial precursor migration in snai1b knockdown embryos. The results provide the molecular mechanism how Snail controls the morphogenesis of heart during embryonic development.

SUBMITTER: Qiao L

PROVIDER: S-EPMC3966048 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Snail modulates the assembly of fibronectin via α5 integrin for myocardial migration in zebrafish embryos.

Qiao Liangjun L Gao Hongwei H Zhang Ting T Jing Lulu L Xiao Chun C Xiao Yue Y Luo Ning N Zhu Hongyan H Meng Wentong W Xu Hong H Mo Xianming X

Scientific reports 20140326

The Snail family member snail encodes a zinc finger-containing transcriptional factor that is involved in heart formation. Yet, little is known about how Snail regulates heart development. Here, we identified that one of the duplicated snail genes, snai1b, was expressed in the heart region of zebrafish embryos. Depletion of Snai1b function dramatically reduced expression of α5 integrin, disrupted Fibronectin layer in the heart region, especially at the midline, and prevented migration of cardiac ...[more]

PMID: 24667151

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