Ontology highlight
ABSTRACT:
SUBMITTER: Merkert S
PROVIDER: S-EPMC3966116 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Merkert Sylvia S Wunderlich Stephanie S Bednarski Christien C Beier Jennifer J Haase Alexandra A Dreyer Anne-Kathrin AK Schwanke Kristin K Meyer Johann J Göhring Gudrun G Cathomen Toni T Martin Ulrich U
Stem cell reports 20140114 1
Genetic engineering of human induced pluripotent stem cells (hiPSCs) via customized designer nucleases has been shown to be significantly more efficient than conventional gene targeting, but still typically depends on the introduction of additional genetic selection elements. In our study, we demonstrate the efficient nonviral and selection-independent gene targeting in human pluripotent stem cells (hPSCs). Our high efficiencies of up to 1.6% of gene-targeted hiPSCs, accompanied by a low backgro ...[more]