Ontology highlight
ABSTRACT:
SUBMITTER: Stergachis AB
PROVIDER: S-EPMC3967546 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Stergachis Andrew B AB Haugen Eric E Shafer Anthony A Fu Wenqing W Vernot Benjamin B Reynolds Alex A Raubitschek Anthony A Ziegler Steven S LeProust Emily M EM Akey Joshua M JM Stamatoyannopoulos John A JA
Science (New York, N.Y.) 20131201 6164
Genomes contain both a genetic code specifying amino acids and a regulatory code specifying transcription factor (TF) recognition sequences. We used genomic deoxyribonuclease I footprinting to map nucleotide resolution TF occupancy across the human exome in 81 diverse cell types. We found that ~15% of human codons are dual-use codons ("duons") that simultaneously specify both amino acids and TF recognition sites. Duons are highly conserved and have shaped protein evolution, and TF-imposed constr ...[more]