Unknown

Dataset Information

0

Potential regulatory role of microRNAs in the development of bovine gastrointestinal tract during early life.


ABSTRACT: This study aimed to investigate the potential regulatory role of miRNAs in the development of gastrointestinal tract (GIT) during the early life of dairy calves. Rumen and small intestinal (mid-jejunum and ileum) tissue samples were collected from newborn (30 min after birth; n?=?3), 7-day-old (n?=?6), 21-day-old (n?=?6), and 42-day-old (n?=?6) dairy calves. The miRNA profiling was performed using Illumina RNA-sequencing and the temporal and regional differentially expressed miRNAs were further validated using qRT-PCR. Analysis of 16S rRNA gene copy numbers was used to quantify total bacteria, Bifidobacterium and Lactobacillus species. The expression of miR-143 was abundant in all three gut regions, at all time points and it targets genes involved primarily in the proliferation of connective tissue cells and muscle cells, suggesting a role in regulating rapid tissue development during the early life of calves. The expression of miR-146, miR-191, miR-33, miR-7, miR-99/100, miR-486, miR-145, miR-196 and miR-211 displayed significant temporal differences (FDR <0.05), while miR-192/215, miR-194, miR-196, miR-205 and miR-31 revealed significant regional differences (FDR <0.05). The expression levels of miR-15/16, miR-29 and miR-196 were positively correlated with the copy numbers of 16S rRNA gene of Bifidobacterium or Lactobacillus species or both (P<0.05). Functional analysis using Ingenuity Pathway Analysis identified the above mentioned differentially expressed miRNAs as potential regulators of gut tissue cell proliferation and differentiation. The bacterial density-associated miRNAs were identified as modulators of the development of lymphoid tissues (miR-196), maturation of dendritic cells (miR-29) and development of immune cells (miR-15/16). The present study revealed temporal and regional changes in miRNA expression and a correlation between miRNA expression and microbial population in the GIT during the early life, which provides further evidence for another mechanism by which host-microbial interactions play a role in regulating gut development.

SUBMITTER: Liang G 

PROVIDER: S-EPMC3969364 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

altmetric image

Publications

Potential regulatory role of microRNAs in the development of bovine gastrointestinal tract during early life.

Liang Guanxiang G   Malmuthuge Nilusha N   McFadden Thomas B TB   Bao Hua H   Griebel Philip J PJ   Stothard Paul P   Guan Le Luo le L  

PloS one 20140328 3


This study aimed to investigate the potential regulatory role of miRNAs in the development of gastrointestinal tract (GIT) during the early life of dairy calves. Rumen and small intestinal (mid-jejunum and ileum) tissue samples were collected from newborn (30 min after birth; n = 3), 7-day-old (n = 6), 21-day-old (n = 6), and 42-day-old (n = 6) dairy calves. The miRNA profiling was performed using Illumina RNA-sequencing and the temporal and regional differentially expressed miRNAs were further  ...[more]

Similar Datasets

2016-10-29 | GSE52193 | GEO
| S-EPMC5816665 | biostudies-literature
| S-EPMC5693515 | biostudies-literature
2012-02-08 | GSE35567 | GEO
2012-02-08 | E-GEOD-35567 | biostudies-arrayexpress
| S-EPMC5759257 | biostudies-literature
| S-EPMC4357424 | biostudies-literature
| S-EPMC3019712 | biostudies-literature
2024-10-15 | GSE241697 | GEO
| S-EPMC4711905 | biostudies-literature