Increased bisecting N-acetylglucosamine and decreased branched chain glycans of N-linked glycoproteins in expressed prostatic secretions associated with prostate cancer progression.

ABSTRACT:

Purpose

Using prostatic fluids rich in glycoproteins like prostate-specific antigen and prostatic acid phosphatase (PAP), the goal of this study was to identify the structural types and relative abundance of glycans associated with prostate cancer status for subsequent use in emerging MS-based glycopeptide analysis platforms.

Experimental design

A series of pooled samples of expressed prostatic secretions (EPS) and exosomes reflecting different stages of prostate cancer disease were used for N-linked glycan profiling by three complementary methods, MALDI-TOF profiling, normal-phase HPLC separation, and triple quadropole MS analysis of PAP glycopeptides.

Results

Glycan profiling of N-linked glycans from different EPS fluids indicated a global decrease in larger branche

SUBMITTER: Nyalwidhe JO 

PROVIDER: S-EPMC3969874 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature