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P54nrb/NonO and PSF promote U snRNA nuclear export by accelerating its export complex assembly.


ABSTRACT: The assembly of spliceosomal U snRNPs in metazoans requires nuclear export of U snRNA precursors. Four factors, nuclear cap-binding complex (CBC), phosphorylated adaptor for RNA export (PHAX), the export receptor CRM1 and RanGTP, gather at the m(7)G-cap-proximal region and form the U snRNA export complex. Here we show that the multifunctional RNA-binding proteins p54nrb/NonO and PSF are U snRNA export stimulatory factors. These proteins, likely as a heterodimer, accelerate the recruitment of PHAX, and subsequently CRM1 and Ran onto the RNA substrates in vitro, which mediates efficient U snRNA export in vivo. Our results reveal a new layer of regulation for U snRNA export and, hence, spliceosomal U snRNP biogenesis.

SUBMITTER: Izumi H 

PROVIDER: S-EPMC3973303 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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p54nrb/NonO and PSF promote U snRNA nuclear export by accelerating its export complex assembly.

Izumi Hiroto H   McCloskey Asako A   Shinmyozu Kaori K   Ohno Mutsuhito M  

Nucleic acids research 20140110 6


The assembly of spliceosomal U snRNPs in metazoans requires nuclear export of U snRNA precursors. Four factors, nuclear cap-binding complex (CBC), phosphorylated adaptor for RNA export (PHAX), the export receptor CRM1 and RanGTP, gather at the m(7)G-cap-proximal region and form the U snRNA export complex. Here we show that the multifunctional RNA-binding proteins p54nrb/NonO and PSF are U snRNA export stimulatory factors. These proteins, likely as a heterodimer, accelerate the recruitment of PHA  ...[more]

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