Ontology highlight
ABSTRACT:
SUBMITTER: Choi YS
PROVIDER: S-EPMC3977815 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
PloS one 20140407 4
We developed a process to produce novel interactions between two previously unrelated proteins. This process selects protein scaffolds and designs protein interfaces that bind to a surface patch of interest on a target protein. Scaffolds with shapes complementary to the target surface patch were screened using an exhaustive computational search of the human proteome and optimized by directed evolution using phage display. This method was applied to successfully design scaffolds that bind to epid ...[more]