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Exploiting human CD34+ stem cell-conditioned medium for tissue repair.

ABSTRACT: Despite the progress in our understanding of genes essential for stem cell regulation and development, little is known about the factors secreted by stem cells and their effect on tissue regeneration. In particular, the factors secreted by human CD34+ cells remain to be elucidated. We have approached this challenge by performing a cytokine/growth factor microarray analysis of secreted soluble factors in medium conditioned by adherent human CD34+ cells. Thirty-two abundantly secreted factors have been identified, all of which are associated with cell proliferation, survival, tissue repair, and wound healing. The cultured CD34+ cells expressed known stem cell genes such as Nanog, Oct4, Sox2, c-kit, and HoxB4. The conditioned medium containing the secreted factors prevented cell death in liver cells exposed to liver toxin in vitro via inhibition of the caspase-3 signaling pathway. More importantly, in vivo studies using animal models of liver damage demonstrated that injection of the conditioned medium could repair damaged liver tissue (significant reduction in the necroinflammatory activity), as well as enable the animals to survive. Thus, we demonstrate that medium conditioned by human CD34+ cells has the potential for therapeutic repair of damaged tissue in vivo.

SUBMITTER: Mintz PJ

PROVIDER: S-EPMC3978788 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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Exploiting human CD34+ stem cell-conditioned medium for tissue repair.

Mintz Paul J PJ Huang Kai-Wen KW Reebye Vikash V Nteliopoulos Georgios G Lai Hong-Shiee HS Sætrom Pal P Kasahara Noriyuki N Jensen Steen S Pai Madhava M Gordon Myrtle Y A MY Marley Stephen B SB Behan Rosemary R Spalding Duncan R DR Haoudi Abdelali A Emara Mohamed M MM Nicholls Joanna J Rossi John J JJ Habib Nagy A NA

Molecular therapy : the journal of the American Society of Gene Therapy 20130828 1

Despite the progress in our understanding of genes essential for stem cell regulation and development, little is known about the factors secreted by stem cells and their effect on tissue regeneration. In particular, the factors secreted by human CD34+ cells remain to be elucidated. We have approached this challenge by performing a cytokine/growth factor microarray analysis of secreted soluble factors in medium conditioned by adherent human CD34+ cells. Thirty-two abundantly secreted factors have ...[more]

PMID: 23985698

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Exploiting human CD34+ stem cell-conditioned medium for tissue repair.

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Exploiting human CD34+ stem cell-conditioned medium for tissue repair.

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