Ontology highlight
ABSTRACT:
SUBMITTER: Qiu Y
PROVIDER: S-EPMC3979297 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Qiu Yupeng Y Antony Edwin E Doganay Sultan S Koh Hye Ran HR Lohman Timothy M TM Myong Sua S
Nature communications 20130101
Srs2 dismantles presynaptic Rad51 filaments and prevents its re-formation as an anti-recombinase. However, the molecular mechanism by which Srs2 accomplishes these tasks remains unclear. Here we report a single-molecule fluorescence study of the dynamics of Rad51 filament formation and its disruption by Srs2. Rad51 forms filaments on single-stranded DNA by sequential binding of primarily monomers and dimers in a 5'-3' direction. One Rad51 molecule binds to three nucleotides, and six monomers are ...[more]