Ontology highlight
ABSTRACT:
SUBMITTER: Masatani T
PROVIDER: S-EPMC3979947 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Masatani Tatsunori T Asada Masahito M Ichikawa-Seki Madoka M Usui Miho M Terkawi Mohamad A MA Hayashi Kei K Kawazu Shin-Ichiro S Xuan Xuenan X
The Journal of veterinary medical science 20130911 1
Peroxiredoxins (Prxs) are a family of antioxidant enzymes. Here, we cloned a 2-Cys Prx, BgTPx-1, from the canine Babesia parasite B. gibsoni. Sequence identity between BgTPx-1 and 2-Cys Prx of B. bovis was 81% at the amino acid level. Enzyme activity assay by using recombinant BgTPx-1 (rBgTPx-1) indicated that BgTPx-1 has antioxidant activity. Antiserum from a mouse immunized with rBgTPx-1 reacted with parasite lysates and detect a protein with a monomeric size of 22 kDa and also a 44 kDa protei ...[more]