Upregulation of DMT1 expression in choroidal epithelia of the blood-CSF barrier following manganese exposure in vitro.

ABSTRACT: Divalent metal transporter 1 (DMT1), whose mRNA possesses a stem-loop structure in 3'-untranslated region, has been identified in most organs and responsible for transport of various divalent metal ions. Previous work from this laboratory has shown that manganese (Mn) exposure alters the function of iron regulatory protein (IRP) and increases iron (Fe) concentrations in the cerebrospinal fluid (CSF). This study was designed to test the hypothesis that Mn treatment, by acting on protein-mRNA binding between IRP and DMT1 mRNA, altered the expression of DMT1 in an immortalized choroidal epithelial Z310 cell line which was derived from rat choroid plexus epithelia, leading to a compartmental shift of Fe from the blood to the CSF. Immunocytochemistry confirmed the presence of DMT1 in Z310 cell.

SUBMITTER: Wang X 

PROVIDER: S-EPMC3980874 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature