Ontology highlight
ABSTRACT:
SUBMITTER: Stafa A
PROVIDER: S-EPMC3982708 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Stafa Anamarija A Donnianni Roberto A RA Timashev Leonid A LA Lam Alicia F AF Symington Lorraine S LS
Genetics 20140204 4
Chromosomal double-strand breaks (DSBs) that have only one end with homology to a donor duplex undergo repair by strand invasion followed by replication to the chromosome terminus (break-induced replication, BIR). Using a transformation-based assay system, it was previously shown that BIR could occur by several rounds of strand invasion, DNA synthesis, and dissociation. Here we describe a modification of the transformation-based assay to facilitate detection of switching between donor templates ...[more]