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Differential regulation of myosin heavy chains defines new muscle domains in zebrafish.

ABSTRACT: Numerous muscle lineages are formed during myogenesis within both slow- and fast-specific cell groups. In this study, we show that six fast muscle-specific myosin heavy chain genes have unique expression patterns in the zebrafish embryo. The expression of tail-specific myosin heavy chain (fmyhc2.1) requires wnt signaling and is essential for fast muscle organization within the tail. Retinoic acid treatment results in reduced wnt signaling, which leads to loss of the fmyhc2.1 domain. Retinoic acid treatment also results in a shift of muscle identity within two trunk domains defined by expression of fmyhc1.2 and fmyhc1.3 in favor of the anteriormost myosin isoform, fmyhc1.2. In summary, we identify new muscle domains along the anteroposterior axis in the zebrafish that are defined by individual nonoverlapping, differentially regulated expression of myosin heavy chain isoforms.

SUBMITTER: Nord H

PROVIDER: S-EPMC3983002 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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Differential regulation of myosin heavy chains defines new muscle domains in zebrafish.

Nord Hanna H Burguiere Anne-Cecile AC Muck Joscha J Nord Christoffer C Ahlgren Ulf U von Hofsten Jonas J

Molecular biology of the cell 20140212 8

Numerous muscle lineages are formed during myogenesis within both slow- and fast-specific cell groups. In this study, we show that six fast muscle-specific myosin heavy chain genes have unique expression patterns in the zebrafish embryo. The expression of tail-specific myosin heavy chain (fmyhc2.1) requires wnt signaling and is essential for fast muscle organization within the tail. Retinoic acid treatment results in reduced wnt signaling, which leads to loss of the fmyhc2.1 domain. Retinoic aci ...[more]

PMID: 24523292

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