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Crystal structure of the trithorax group protein ASH2L reveals a forkhead-like DNA binding domain.

ABSTRACT: Absent, small or homeotic discs-like 2 (ASH2L) is a trithorax group (TrxG) protein and a regulatory subunit of the SET1 family of lysine methyltransferases. Here we report that ASH2L binds DNA using a forkhead-like helix-wing-helix (HWH) domain. In vivo, the ASH2L HWH domain is required for binding to the ?-globin locus control region, histone H3 Lys4 (H3K4) trimethylation and maximal expression of the ?-globin gene (Hbb-1), validating the functional importance of the ASH2L DNA binding domain.

SUBMITTER: Sarvan S

PROVIDER: S-EPMC3983046 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Crystal structure of the trithorax group protein ASH2L reveals a forkhead-like DNA binding domain.

Sarvan Sabina S Avdic Vanja V Tremblay Véronique V Chaturvedi Chandra-Prakash CP Zhang Pamela P Lanouette Sylvain S Blais Alexandre A Brunzelle Joseph S JS Brand Marjorie M Couture Jean-François JF

Nature structural & molecular biology 20110605 7

Absent, small or homeotic discs-like 2 (ASH2L) is a trithorax group (TrxG) protein and a regulatory subunit of the SET1 family of lysine methyltransferases. Here we report that ASH2L binds DNA using a forkhead-like helix-wing-helix (HWH) domain. In vivo, the ASH2L HWH domain is required for binding to the β-globin locus control region, histone H3 Lys4 (H3K4) trimethylation and maximal expression of the β-globin gene (Hbb-1), validating the functional importance of the ASH2L DNA binding domain. ...[more]

PMID: 21642971

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