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Production of bioactive compounds by actinomycetes and their antioxidant properties.

ABSTRACT: An actinomycete was isolated from mangrove soil collected from Nellore region of Andhra Pradesh, India, and screened for its ability to produce bioactive compounds. The cultural, morphological, and biochemical characters and 16S rRNA sequencing suggest that the isolated strain is Nocardiopsis alba. The bioactive compounds produced by this strain were purified by column chromatography. The in vitro antioxidant capacity of the isolated compounds (fractions) was estimated and fraction F2 showed very near values to the standard ascorbic acid. The potential fraction obtained by column chromatography was subjected to HPLC for further purification, then this purified fraction F2 was examined by FTIR, NMR, and mass spectroscopy to elucidate its chemical structure. By spectral data, the structure of the isolated compound was predicted as "(Z)-1-((1-hydroxypenta-2,4-dien-1-yl)oxy)anthracene-9,10-dione."

SUBMITTER: Janardhan A

PROVIDER: S-EPMC3984769 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Production of bioactive compounds by actinomycetes and their antioxidant properties.

Janardhan Avilala A Kumar Arthala Praveen AP Viswanath Buddolla B Saigopal D V R DV Narasimha Golla G

Biotechnology research international 20140326

An actinomycete was isolated from mangrove soil collected from Nellore region of Andhra Pradesh, India, and screened for its ability to produce bioactive compounds. The cultural, morphological, and biochemical characters and 16S rRNA sequencing suggest that the isolated strain is Nocardiopsis alba. The bioactive compounds produced by this strain were purified by column chromatography. The in vitro antioxidant capacity of the isolated compounds (fractions) was estimated and fraction F2 showed ver ...[more]

PMID: 24790761

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