Ontology highlight
ABSTRACT:
SUBMITTER: Goldberg JM
PROVIDER: S-EPMC3985465 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20140128 5
Thioamide quenchers can be paired with compact fluorophores to design "turn-on" fluorescent protease substrates. We have used this method to study a variety of serine-, cysteine-, carboxyl-, and metallo-proteases, including trypsin, chymotrypsin, pepsin, thermolysin, papain, and calpain. Since thioamides quench some fluorophores red-shifted from those naturally occurring in proteins, this technique can be used for real time monitoring of protease activity in crude preparations of virtually any p ...[more]