A reprogrammable mouse strain from gene-targeted embryonic stem cells.
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ABSTRACT:
SUBMITTER: Stadtfeld M
PROVIDER: S-EPMC3987893 | biostudies-literature | 2010 Jan
REPOSITORIES: biostudies-literature
Publications
A reprogrammable mouse strain from gene-targeted embryonic stem cells.
Stadtfeld Matthias M Maherali Nimet N Borkent Marti M Hochedlinger Konrad K
Nature methods 20091213 1
The derivation of induced pluripotent stem cells (iPSCs) usually involves the viral introduction of reprogramming factors into somatic cells. Here we used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. As these 'reprogrammable mice' can be easily bred, they are a useful tool to study the mechanisms underlying cellular reprogramming. ...[more]
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