Improvements to bead-based oligonucleotide ligation SNP genotyping assays.
Ontology highlight
ABSTRACT:
SUBMITTER: Bruse S
PROVIDER: S-EPMC3987951 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Publications
Improvements to bead-based oligonucleotide ligation SNP genotyping assays.
Bruse Shannon S Moreau Michael M Azaro Marco M Zimmerman Ray R Brzustowicz Linda L
BioTechniques 20081101 5
We describe a bead-based, multiplexed, oligonucleotide ligation assay (OLA) performed on the Luminex flow cytometer. Differences between this method and those previously reported include the use of far fewer beads and the use of a universal oligonucleotide for signal detection. These innovations serve to significantly reduce the cost of the assay, while maintaining robustness and accuracy. Comparisons are made between the Luminex OLA and both pyrosequencing and direct sequencing. Experiments to ...[more]
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