Ontology highlight
ABSTRACT:
SUBMITTER: Yu Z
PROVIDER: S-EPMC3988796 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Yu Zhongsheng Z Chen Hanqing H Liu Jiyong J Zhang Hongtao H Yan Yan Y Zhu Nannan N Guo Yawen Y Yang Bo B Chang Yan Y Dai Fei F Liang Xuehong X Chen Yixu Y Shen Yan Y Deng Wu-Min WM Chen Jianming J Zhang Bo B Li Changqing C Jiao Renjie R
Biology open 20140415 4
Modifying the genomes of many organisms is becoming as easy as manipulating DNA in test tubes, which is made possible by two recently developed techniques based on either the customizable DNA binding protein, TALEN, or the CRISPR/Cas9 system. Here, we describe a series of efficient applications derived from these two technologies, in combination with various homologous donor DNA plasmids, to manipulate the Drosophila genome: (1) to precisely generate genomic deletions; (2) to make genomic replac ...[more]