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Vitamin E, ?-tocopherol, diminishes ex vivo basophil response to dust mite allergen.

ABSTRACT: Epidemiologic studies suggest that dietary vitamin E is a candidate intervention for atopic disease. We used in vitro and ex vivo exposures to test the hypothesis that the most common dietary isoform of vitamin E, ?-tocopherol (?T), could suppress Fc?RI-mediated basophil activation. Rat basophilic leukemia (RBL)-SX38 cells that express human Fc?RI were treated with or without ?T, followed by stimulation with ?-IgE. In the ex vivo study, 20 Der f 1-allergic volunteers consumed a ?T-enriched supplement for 7 days. Their basophils were challenged ex vivo with ?-IgE and graded doses of Der f 1 before and after the supplementation period. ?t treatment of RBL-SX38 cells significantly reduced basophil degranulation and de novo TH2 cytokine production. Daily consumption of a ?T-rich supplement by dust mite-allergic volunteers reduced basophil activation after ex vivo dust mite challenge. Vitamin E supplements rich in ?T may be useful adjuncts in decreasing atopic disease.

SUBMITTER: Mills K

PROVIDER: S-EPMC3989399 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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Vitamin E, γ-tocopherol, diminishes ex vivo basophil response to dust mite allergen.

Mills K K Lay J J Wu W W Robinette C C Kesic M J MJ Dreskin S C SC Peden D B DB Hernandez M M

Allergy 20140401 4

Epidemiologic studies suggest that dietary vitamin E is a candidate intervention for atopic disease. We used in vitro and ex vivo exposures to test the hypothesis that the most common dietary isoform of vitamin E, γ-tocopherol (γT), could suppress FcεRI-mediated basophil activation. Rat basophilic leukemia (RBL)-SX38 cells that express human FcεRI were treated with or without γT, followed by stimulation with α-IgE. In the ex vivo study, 20 Der f 1-allergic volunteers consumed a γT-enriched suppl ...[more]

PMID: 24697338

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Project description:BackgroundAllergen immunotherapy (AIT) is a specific treatment of administering clinically important allergens to patients who have allergic diseases. In Japan, the standardized house dust mite (HDM) allergen for subcutaneous immunotherapy (SCIT) was approved in 2015, and we then introduced rush-immunotherapy (rush-IT) using the standardized HDM allergen for HDM-sensitive asthmatics. However, little data are available on the safety and effectiveness of rush-HDM-IT, especially for Japanese asthmatics.ObjectiveThe objective of this study was to examine the safety and clinical effectiveness of rush-IT using the standardized HDM for HDM-sensitive Japanese asthmatics.MethodsThirteen HDM-sensitive asthmatics who received rush-HDM-IT and 12 HDM-sensitive asthmatic controls were enrolled. To evaluate the safety, the number of systemic reaction (SR) events, including anaphylaxis, was assessed. To evaluate the effectiveness, changes in the treatment step, dose of inhaled corticosteroid, and asthma control after rush-HDM-IT and the subsequent maintenance SCIT were assessed. Changes in the HDM-induced production of type 2 cytokines from peripheral blood mononuclear cells were also evaluated.ResultsAmong the 12 patients who received rush-IT, 4 (30.7%) experienced a SR and 3 (23.1%) experienced anaphylaxis. However, the anaphylaxis was not severe (grade 3) in all cases, and they recovered in a short time. The treatment step of asthma was better and the dose of inhaled corticosteroid was lower in the rush-HDM-IT group than in the control group. The HDM-induced production of both interleukin (IL)-5 and IL-13 from peripheral blood mononuclear cells was significantly lower in the rush-HDM-IT group than in the control group.ConclusionRush-HDM-IT can be performed relatively safely in Japanese asthmatics. Furthermore, rush-HDM-IT and the subsequent maintenance SCIT provided clinical improvement in asthma patients, and was accompanied by the suppression of HDM-specific Th2-mediated systemic immune responses.

Dataset Information

Vitamin E, ?-tocopherol, diminishes ex vivo basophil response to dust mite allergen.

Publications

Vitamin E, γ-tocopherol, diminishes ex vivo basophil response to dust mite allergen.

Similar Datasets

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