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M13 virus based detection of bacterial infections in living hosts.

ABSTRACT: We report a first method for using M13 bacteriophage as a multifunctional scaffold for optically imaging bacterial infections in vivo. We demonstrate that M13 virus conjugated with hundreds of dye molecules (M13-Dye) can target and distinguish pathogenic infections of F-pili expressing and F-negative strains of E. coli. Further, in order to tune this M13-Dye complex suitable for targeting other strains of bacteria, we have used a 1-step reaction for creating an anti-bacterial antibody-M13-Dye probe. As an example, we show anti-S. aureus-M13-Dye able to target and image infections of S. aureus in living hosts, with a 3.7× increase in fluorescence over background.

SUBMITTER: Bardhan NM

PROVIDER: S-EPMC3989466 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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M13 virus based detection of bacterial infections in living hosts.

Bardhan Neelkanth M NM Ghosh Debadyuti D Belcher Angela M AM

Journal of biophotonics 20130411 8

We report a first method for using M13 bacteriophage as a multifunctional scaffold for optically imaging bacterial infections in vivo. We demonstrate that M13 virus conjugated with hundreds of dye molecules (M13-Dye) can target and distinguish pathogenic infections of F-pili expressing and F-negative strains of E. coli. Further, in order to tune this M13-Dye complex suitable for targeting other strains of bacteria, we have used a 1-step reaction for creating an anti-bacterial antibody-M13-Dye pr ...[more]

PMID: 23576418

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