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H2B ubiquitylation modulates spliceosome assembly and function in budding yeast.

ABSTRACT: Commitment to splicing occurs co-transcriptionally, but a major unanswered question is the extent to which various modifications of chromatin, the template for transcription in vivo, contribute to the regulation of splicing.Here, we perform genome-wide analyses showing that inhibition of specific marks - H2B ubiquitylation, H3K4 methylation and H3K36 methylation - perturbs splicing in budding yeast, with each modification exerting gene-specific effects. Furthermore, semi-quantitative mass spectrometry on purified nuclear mRNPs and chromatin immunoprecipitation analysis on intron-containing genes indicated that H2B ubiquitylation, but not Set1-, Set2- or Dot1-dependent H3 methylation, stimulates recruitment of the early splicing factors, namely U1 and U2 snRNPs, onto nascent RNAs.These results suggest that histone modifications impact splicing of distinct subsets of genes using distinct pathways.

SUBMITTER: Herissant L

PROVIDER: S-EPMC3989899 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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H2B ubiquitylation modulates spliceosome assembly and function in budding yeast.

Hérissant Lucas L Moehle Erica A EA Bertaccini Diego D Van Dorsselaer Alain A Schaeffer-Reiss Christine C Guthrie Christine C Dargemont Catherine C

Biology of the cell 20140225 4

<h4>Background information</h4>Commitment to splicing occurs co-transcriptionally, but a major unanswered question is the extent to which various modifications of chromatin, the template for transcription in vivo, contribute to the regulation of splicing.<h4>Results</h4>Here, we perform genome-wide analyses showing that inhibition of specific marks - H2B ubiquitylation, H3K4 methylation and H3K36 methylation - perturbs splicing in budding yeast, with each modification exerting gene-specific effe ...[more]

PMID: 24476359

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H2B ubiquitylation is part of chromatin architecture that marks exon-intron structure in budding yeast.

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