Ontology highlight
ABSTRACT:
SUBMITTER: Dow LE
PROVIDER: S-EPMC3990578 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Dow Lukas E LE Nasr Zeina Z Saborowski Michael M Ebbesen Saya H SH Manchado Eusebio E Tasdemir Nilgun N Lee Teresa T Pelletier Jerry J Lowe Scott W SW
PloS one 20140417 4
Tetracycline or doxycycline (dox)-regulated control of genetic elements allows inducible, reversible and tissue specific regulation of gene expression in mice. This approach provides a means to investigate protein function in specific cell lineages and at defined periods of development and disease. Efficient and stable regulation of cDNAs or non-coding elements (e.g. shRNAs) downstream of the tetracycline-regulated element (TRE) requires the robust expression of a tet-transactivator protein, com ...[more]