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A screen for short-range paracrine interactions.

ABSTRACT: Conventional methods for studying paracrine signaling in vitro may not be sensitive to short-range effects resulting from signal dilution or decay. We employ a microfabricated culture substrate to maintain two cell populations in microscale proximity. Individual populations can be quickly retrieved for cell-specific readouts by standard high-throughput assays. We show that this platform is sensitive to short-range interactions that are not detectable by common methods such as conditioned media transfer or porous cell culture inserts, as revealed by gene expression changes in a tumor-stromal crosstalk model. In addition, we are able to detect population-specific gene expression changes that would have been masked in mixed co-cultures. We thus demonstrate a tool for investigating an important class of intercellular communication that may be overlooked in conventional biological studies.

SUBMITTER: Spencer KH

PROVIDER: S-EPMC3991816 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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A screen for short-range paracrine interactions.

Spencer K H KH Kim M Y MY Hughes C C W CC Hui E E EE

Integrative biology : quantitative biosciences from nano to macro 20140213 4

Conventional methods for studying paracrine signaling in vitro may not be sensitive to short-range effects resulting from signal dilution or decay. We employ a microfabricated culture substrate to maintain two cell populations in microscale proximity. Individual populations can be quickly retrieved for cell-specific readouts by standard high-throughput assays. We show that this platform is sensitive to short-range interactions that are not detectable by common methods such as conditioned media t ...[more]

PMID: 24522172

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